› 4ctd

X-ray diffraction
3.2Å resolution

X-ray structure of an engineered OmpG loop6-deletion

Released:
DOI: 10.2210/pdb4ctd/pdb
PDB entry 4ctd coloured by chain, front view.
PDB entry 4ctd coloured by chain, side view.
PDB entry 4ctd coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structure-based engineering of a minimal porin reveals loop-independent channel closure.
Grosse W, Psakis G, Mertins B, Reiss P, Windisch D, Brademann F, Bürck J, Ulrich A, Koert U, Essen LO
Biochemistry 53 4826-38 (2014)
PMID: 24988371

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160040 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Outer membrane porin G Chains: A, B
Molecule details ›
Chains: A, B
Length: 274 amino acids
Theoretical weight: 31.94 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P76045 (Residues: 22-301; Coverage: 96%)
Gene names: JW1312, b1319, ompG
Sequence domains: Outer membrane protein G (OmpG)
Structure domains: monomeric porin ompg

Ligands and Environments

2 bound ligands:
Ligand CL 12 x CL
Ligand C8E 4 x C8E
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P1
Unit cell:
a: 59.94Å b: 60.06Å c: 59.76Å
α: 70.45° β: 95.24° γ: 70.58°
R-values:
R R work R free
0.241 0.238 0.289
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Bcl-2-Protein Family as Modulators of IP3 Receptors and Other Organellar Ca2+ Channels.
Ivanova et al. (2020)
3 more

4 mentions without citation

Integrin α6β4 in Colorectal Cancer: Expression, Regulation, Functional Alterations and Use as a Biomarker.
Beaulieu JF. (2019)
3 more