› 4cth

X-ray diffraction
2.15Å resolution

Neprilysin variant G399V,G714K in complex with phosphoramidon

Released:
DOI: 10.2210/pdb4cth/pdb
PDB entry 4cth coloured by chain, front view.
PDB entry 4cth coloured by chain, side view.
PDB entry 4cth coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Engineering neprilysin activity and specificity to create a novel therapeutic for Alzheimer's disease.
Webster CI, Burrell M, Olsson LL, Fowler SB, Digby S, Sandercock A, Snijder A, Tebbe J, Haupts U, Grudzinska J, Jermutus L, Andersson C
PLoS One 9 e104001 (2014)
PMID: 25089527

Function and Biology Details

Reaction catalysed: 
Preferential cleavage of polypeptides between hydrophobic residues, particularly with Phe or Tyr at P1'.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140131 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Neprilysin Chain: A
Molecule details ›
Chain: A
Length: 709 amino acids
Theoretical weight: 81.41 KDa
Source organism: Homo sapiens
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P08473 (Residues: 52-750; Coverage: 93%)
Gene names: EPN, MME
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand NAG 3 x NAG
Ligand ZN 1 x ZN
Ligand GOL 8 x GOL
Ligand RDF 1 x RDF
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P3221
Unit cell:
a: 108.799Å b: 108.799Å c: 112.942Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.212 0.21 0.261
Expression system: Saccharomyces cerevisiae

Quick links

Citations

11 review citations

A Test in Context: Neprilysin: Function, Inhibition, and Biomarker.
Bayes-Genis et al. (2016)
10 more

2 mentions without citation

Engineering neprilysin activity and specificity to create a novel therapeutic for Alzheimer's disease.
Webster et al. (2014)
1 more