› 4cvh

X-ray diffraction
2.39Å resolution

Crystal structure of human isoprenoid synthase domain-containing protein

Released:
DOI: 10.2210/pdb4cvh/pdb
PDB entry 4cvh coloured by chain, front view.
PDB entry 4cvh coloured by chain, side view.
PDB entry 4cvh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Human ISPD Is a Cytidyltransferase Required for Dystroglycan O-Mannosylation.
Riemersma M, Froese DS, van Tol W, Engelke UF, Kopec J, van Scherpenzeel M, Ashikov A, Krojer T, von Delft F, Tessari M, Buczkowska A, Swiezewska E, Jae LT, Brummelkamp TR, Manya H, Endo T, van Bokhoven H, Yue WW, Lefeber DJ
Chem Biol 22 1643-52 (2015)
PMID: 26687144

Function and Biology Details

Reaction catalysed: 
CTP + D-ribitol 5-phosphate = diphosphate + CDP-ribitol
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107260 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-ribitol-5-phosphate cytidylyltransferase Chain: A
Molecule details ›
Chain: A
Length: 411 amino acids
Theoretical weight: 46.11 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: A4D126 (Residues: 43-451; Coverage: 91%)
Gene names: CRPPA, ISPD
Sequence domains:
Structure domains: Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A

Ligands and Environments

3 bound ligands:
Ligand MG 1 x MG
Ligand EDO 1 x EDO
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: C2221
Unit cell:
a: 79.36Å b: 116.8Å c: 111.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.189 0.244
Expression system: Spodoptera frugiperda

Quick links

Citations

13 review citations

Biological roles of glycans.
Varki A. (2017)
12 more

4 mentions without citation

From structural biology to designing therapy for inborn errors of metabolism.
Yue WW. (2016)
3 more