› 4cvk

X-ray diffraction
1.92Å resolution

PaMurF in complex with UDP-MurNAc-tripeptide (mDAP)

Released:
DOI: 10.2210/pdb4cvk/pdb
PDB entry 4cvk coloured by chain, front view.
PDB entry 4cvk coloured by chain, side view.
PDB entry 4cvk coloured by chain, top view.
Source organisms:
Entry authors: Ruane KM, Majce V

Function and Biology Details

Reaction catalysed: 
ATP + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine + D-alanyl-D-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164355 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase Chain: A
Molecule details ›
Chain: A
Length: 475 amino acids
Theoretical weight: 49.37 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HVZ7 (Residues: 1-458; Coverage: 100%)
Gene names: PA4416, murF
Sequence domains:
Structure domains:
ALA-FGA-API Chain: B
Molecule details ›
Chain: B
Length: 3 amino acids
Theoretical weight: 390 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand UDP 1 x UDP
Ligand MUB 1 x MUB
2 modified residues:
Ligand FGA 1 x FGA
Ligand API 1 x API

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P212121
Unit cell:
a: 57.81Å b: 87.29Å c: 92.61Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.196 0.226
Expression systems:
  • Escherichia coli
  • Not provided

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