› 4cvx

X-ray diffraction
3.3Å resolution

COMPLEX OF A B2 CHICKEN MHC CLASS I MOLECULE AND A 9MER CHICKEN PEPTIDE

Released:
DOI: 10.2210/pdb4cvx/pdb
PDB entry 4cvx coloured by chain, front view.
PDB entry 4cvx coloured by chain, side view.
PDB entry 4cvx coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Expression levels of MHC class I molecules are inversely correlated with promiscuity of peptide binding.
Chappell P, Meziane el K, Harrison M, Magiera Ł, Hermann C, Mears L, Wrobel AG, Durant C, Nielsen LL, Buus S, Ternette N, Mwangi W, Butter C, Nair V, Ahyee T, Duggleby R, Madrigal A, Roversi P, Lea SM, Kaufman J
Elife 4 e05345 (2015)
PMID: 25860507

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-129226 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ig-like domain-containing protein Chains: A, D
Molecule details ›
Chains: A, D
Length: 310 amino acids
Theoretical weight: 35.35 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O46789 (Residues: 22-293; Coverage: 81%)
Gene names: B-FIV, BF2
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, E
Molecule details ›
Chains: B, E
Length: 98 amino acids
Theoretical weight: 11.06 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P21611 (Residues: 22-119; Coverage: 100%)
Gene name: B2M
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Vacuolar protein sorting 45 homolog Chains: C, F
Molecule details ›
Chains: C, F
Length: 9 amino acids
Theoretical weight: 1.04 KDa
Source organism: Gallus gallus
Expression system: Not provided
UniProt:
  • Canonical: Q5ZJG4 (Residues: 314-322; Coverage: 2%)
Gene name: RCJMB04_18g1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P65
Unit cell:
a: 173.85Å b: 173.85Å c: 87.51Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.238 0.237 0.262
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

18 review citations

A cold-blooded view of adaptive immunity.
Flajnik MF. (2018)
17 more

4 mentions without citation

New vistas unfold: Chicken MHC molecules reveal unexpected ways to present peptides to the immune system.
Halabi et al. (2022)
3 more