› 4cwc

X-ray diffraction
2.9Å resolution

Structure of Rolling Circle Replication Initiator Protein (RepDE) from Staphylococcus aureus

Released:
DOI: 10.2210/pdb4cwc/pdb
PDB entry 4cwc coloured by chain, front view.
PDB entry 4cwc coloured by chain, side view.
PDB entry 4cwc coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Structures of replication initiation proteins from staphylococcal antibiotic resistance plasmids reveal protein asymmetry and flexibility are necessary for replication.
Carr SB, Phillips SE, Thomas CD
Nucleic Acids Res 44 2417-28 (2016)
PMID: 26792891

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136495 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Replication initiation protein Chains: A, C
Molecule details ›
Chains: A, C
Length: 281 amino acids
Theoretical weight: 34.28 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P03065 (Residues: 32-226; Coverage: 63%)
  • Canonical: P12053 (Residues: 230-314; Coverage: 27%)
Gene names: repD, repE
Sequence domains: Replication initiation factor

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: C2
Unit cell:
a: 240.3Å b: 56.536Å c: 62.357Å
α: 90° β: 102.22° γ: 90°
R-values:
R R work R free
0.219 0.216 0.259
Expression system: Escherichia coli

Quick links

Citations

2 review citations

The Different Faces of Rolling-Circle Replication and Its Multifunctional Initiator Proteins.
Wawrzyniak et al. (2017)
1 more

4 mentions without citation

The Facts and Family Secrets of Plasmids That Replicate via the Rolling-Circle Mechanism.
Garcillán-Barcia et al. (2022)
3 more