› 4cxw

X-ray diffraction
3.1Å resolution

Crystal structure of human FTO in complex with subfamily-selective inhibitor 12

Released:
DOI: 10.2210/pdb4cxw/pdb
PDB entry 4cxw coloured by chain, front view.
PDB entry 4cxw coloured by chain, side view.
PDB entry 4cxw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A strategy based on nucleotide specificity leads to a subfamily-selective and cell-active inhibitor of N6-methyladenosine demethylase FTO.
Toh JDW, Sun L, Lau LZM, Tan J, Low JJA, Tang CWQ, Cheong EJY, Tan MJH, Chen Y, Hong W, Gao YG, Woon ECY
Chem Sci 6 112-122 (2015)
PMID: 28553460

Function and Biology Details

Reaction catalysed: 
N(6)-methyladenosine in mRNA + 2-oxoglutarate + O(2) = adenosine in mRNA + formaldehyde + succinate + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189888 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-ketoglutarate-dependent dioxygenase FTO Chain: A
Molecule details ›
Chain: A
Length: 495 amino acids
Theoretical weight: 56.82 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9C0B1 (Residues: 32-505; Coverage: 94%)
Gene names: FTO, KIAA1752
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand NI 1 x NI
Ligand 6MK 1 x 6MK
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: R3
Unit cell:
a: 141.613Å b: 141.613Å c: 83.812Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.184 0.181 0.245
Expression system: Escherichia coli BL21

Quick links

Citations

19 review citations

m6A modification: recent advances, anticancer targeted drug discovery and beyond.
Deng et al. (2022)
18 more

5 mentions without citation

Recent Advances of m6A Demethylases Inhibitors and Their Biological Functions in Human Diseases.
You et al. (2022)
4 more