› 4cza

X-ray diffraction
3.2Å resolution

Structure of the sodium proton antiporter PaNhaP from Pyrococcus abyssii with bound thallium ion.

Released:
DOI: 10.2210/pdb4cza/pdb
PDB entry 4cza coloured by chain, front view.
PDB entry 4cza coloured by chain, side view.
PDB entry 4cza coloured by chain, top view.
Source organism: Pyrococcus abyssi GE5
Primary publication:
Structure and substrate ion binding in the sodium/proton antiporter PaNhaP.
Wöhlert D, Kühlbrandt W, Yildiz O
Elife 3 e03579 (2014)
PMID: 25426802

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194141 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cation/H+ exchanger domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 420 amino acids
Theoretical weight: 46.74 KDa
Source organism: Pyrococcus abyssi GE5
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UZ55 (Residues: 1-420; Coverage: 95%)
Gene name: PAB1518
Sequence domains: Sodium/hydrogen exchanger family

Ligands and Environments

5 bound ligands:
Ligand TAM 4 x TAM
Ligand BOG 1 x BOG
Ligand TL 2 x TL
Ligand UND 1 x UND
Ligand ACT 1 x ACT
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 54.056Å b: 107.402Å c: 99.796Å
α: 90° β: 96.37° γ: 90°
R-values:
R R work R free
0.251 0.248 0.298
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Shared Molecular Mechanisms of Membrane Transporters.
Drew et al. (2016)
7 more

14 mentions without citation

Elevator-type mechanisms of membrane transport.
Garaeva et al. (2020)
13 more