› 4d0e

X-ray diffraction
1.61Å resolution

Human Notch1 EGF domains 11-13 mutant GlcNAc-fucose disaccharide modified at T466

Released:
DOI: 10.2210/pdb4d0e/pdb
PDB entry 4d0e coloured by chain, front view.
PDB entry 4d0e coloured by chain, side view.
PDB entry 4d0e coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Fringe-mediated extension of O-linked fucose in the ligand-binding region of Notch1 increases binding to mammalian Notch ligands.
Taylor P, Takeuchi H, Sheppard D, Chillakuri C, Lea SM, Haltiwanger RS, Handford PA
Proc Natl Acad Sci U S A 111 7290-5 (2014)
PMID: 24803430

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155479 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Neurogenic locus notch homolog protein 1 Chain: A
Molecule details ›
Chain: A
Length: 135 amino acids
Theoretical weight: 14.86 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P46531 (Residues: 411-526; Coverage: 5%)
Gene names: NOTCH1, TAN1
Sequence domains:
Structure domains: Laminin

Ligands and Environments

Carbohydrate polymer : NEW Components: FUC, NAG
Carbohydrate polymer
1 bound ligand:
Ligand CA 4 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P3121
Unit cell:
a: 28.44Å b: 28.44Å c: 282.39Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.232 0.231 0.27
Expression system: Escherichia coli

Quick links

Citations

14 review citations

The notch ligand JAGGED1 as a target for anti-tumor therapy.
Li et al. (2014)
13 more

8 mentions without citation

Protein O-fucosylation: structure and function.
Holdener et al. (2019)
7 more