› 4d0m

X-ray diffraction
6Å resolution

Phosphatidylinositol 4-kinase III beta in a complex with Rab11a-GTP- gamma-S and the Rab-binding domain of FIP3

Released:
DOI: 10.2210/pdb4d0m/pdb
PDB entry 4d0m coloured by chain, front view.
PDB entry 4d0m coloured by chain, side view.
PDB entry 4d0m coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structures of PI4KIIIβ complexes show simultaneous recruitment of Rab11 and its effectors.
Burke JE, Inglis AJ, Perisic O, Masson GR, McLaughlin SH, Rutaganira F, Shokat KM, Williams RL
Science 344 1035-8 (2014)
PMID: 24876499

Function and Biology Details

Reactions catalysed: 
ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
PDBe Complex ID:
PDB-CPX-130862 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Phosphatidylinositol 4-kinase beta Chains: A, C, G, I, M, O, Q, S, W, Y, c, g
Molecule details ›
Chains: A, C, G, I, M, O, Q, S, W, Y, c, g
Length: 566 amino acids
Theoretical weight: 64.59 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9UBF8 (Residues: 121-799; Coverage: 69%)
Gene names: PI4KB, PIK4CB
Sequence domains:
Ras-related protein Rab-11A Chains: B, D, H, J, N, P, R, T, X, Z, d, h
Molecule details ›
Chains: B, D, H, J, N, P, R, T, X, Z, d, h
Length: 219 amino acids
Theoretical weight: 24.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P62491 (Residues: 1-216; Coverage: 100%)
Gene names: RAB11, RAB11A
Sequence domains: Ras family
Rab11 family-interacting protein 3 Chains: E, F, K, L, U, V, a, b, e, f, i, j
Molecule details ›
Chains: E, F, K, L, U, V, a, b, e, f, i, j
Length: 48 amino acids
Theoretical weight: 5.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O75154 (Residues: 713-756; Coverage: 6%)
Gene names: ARFO1, KIAA0665, RAB11FIP3
Sequence domains: FIP domain

Ligands and Environments

3 bound ligands:
Ligand 093 12 x 093
Ligand GSP 12 x GSP
Ligand MG 12 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 199.504Å b: 134.465Å c: 294.328Å
α: 90° β: 90.33° γ: 90°
R-values:
R R work R free
0.259 0.253 0.359
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli BL21(DE3)

Quick links

Citations

21 review citations

Recommendations for performing, interpreting and reporting hydrogen deuterium exchange mass spectrometry (HDX-MS) experiments.
Masson et al. (2019)
20 more

3 mentions without citation

Rab GTPases and their interacting protein partners: Structural insights into Rab functional diversity.
Pylypenko et al. (2018)
2 more