› 4d2d

X-ray diffraction
2.52Å resolution

Structure of a tri peptide bound POT family peptide transporter

Released:
DOI: 10.2210/pdb4d2d/pdb
PDB entry 4d2d coloured by chain, front view.
PDB entry 4d2d coloured by chain, side view.
PDB entry 4d2d coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for polyspecificity in the POT family of proton-coupled oligopeptide transporters.
Lyons JA, Parker JL, Solcan N, Brinth A, Li D, Shah ST, Caffrey M, Newstead S
EMBO Rep 15 886-93 (2014)
PMID: 24916388

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164364 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Major facilitator superfamily (MFS) profile domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 491 amino acids
Theoretical weight: 53.72 KDa
Source organism: Streptococcus thermophilus LMG 18311
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5M4H8 (Residues: 1-483; Coverage: 100%)
Gene names: dtpT, stu0970
Sequence domains: POT family
Structure domains: MFS general substrate transporter like domains
ALANINE-TRIPEPTIDE Chain: B
Molecule details ›
Chain: B
Length: 3 amino acids
Theoretical weight: 231 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand 78M 3 x 78M
Ligand 78N 3 x 78N
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: C2221
Unit cell:
a: 103.38Å b: 110.7Å c: 110.62Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.198 0.242
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

7 review citations

Shared Molecular Mechanisms of Membrane Transporters.
Drew et al. (2016)
6 more

13 mentions without citation

A comprehensive review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes.
Caffrey M. (2015)
12 more