› 4d49

X-ray diffraction
2.09Å resolution

Crystal structure of computationally designed armadillo repeat proteins for modular peptide recognition.

Released:
DOI: 10.2210/pdb4d49/pdb
PDB entry 4d49 coloured by chain, front view.
PDB entry 4d49 coloured by chain, side view.
PDB entry 4d49 coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Computationally Designed Armadillo Repeat Proteins for Modular Peptide Recognition.
Reichen C, Hansen S, Forzani C, Honegger A, Fleishman SJ, Zhou T, Parmeggiani F, Ernst P, Madhurantakam C, Ewald C, Mittl PRE, Zerbe O, Baker D, Caflisch A, Plückthun A
J Mol Biol 428 4467-4489 (2016)
PMID: 27664438

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164368 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
ARMADILLO REPEAT PROTEIN ARM00027 Chains: A, B, E, F
Molecule details ›
Chains: A, B, E, F
Length: 243 amino acids
Theoretical weight: 25.85 KDa
Source organism: synthetic construct
Expression system: Escherichia coli K-12
Structure domains: Leucine-rich Repeat Variant
POLY ARG DECAPEPTIDE Chains: C, D, G, H
Molecule details ›
Chains: C, D, G, H
Length: 10 amino acids
Theoretical weight: 1.59 KDa
Source organism: synthetic construct
Expression system: Escherichia coli K-12

Ligands and Environments

1 bound ligand:
Ligand ARG 2 x ARG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 88.57Å b: 51.73Å c: 107.61Å
α: 90° β: 90.16° γ: 90°
R-values:
R R work R free
0.204 0.201 0.255
Expression system: Escherichia coli K-12

Quick links

Citations

3 review citations

Designing repeat proteins: a modular approach to protein design.
Parmeggiani et al. (2017)
2 more

1 mention without citation

Global DNA methylation profiling reveals silencing of a secreted form of Epha7 in mouse and human germinal center B-cell lymphomas.
Dawson et al. (2007)