› 4d50

X-ray diffraction
1.7Å resolution

Structure of human deoxyhypusine hydroxylase

Released:
DOI: 10.2210/pdb4d50/pdb
PDB entry 4d50 coloured by chain, front view.
PDB entry 4d50 coloured by chain, side view.
PDB entry 4d50 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal Structure of the Peroxo-diiron(III) Intermediate of Deoxyhypusine Hydroxylase, an Oxygenase Involved in Hypusination.
Han Z, Sakai N, Böttger LH, Klinke S, Hauber J, Trautwein AX, Hilgenfeld R
Structure 23 882-892 (2015)
PMID: 25865244

Function and Biology Details

Reaction catalysed: 
[eIF5A]-deoxyhypusine + AH(2) + O(2) = [eIF5A]-hypusine + A + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189729 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Deoxyhypusine hydroxylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 294 amino acids
Theoretical weight: 31.99 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9BU89 (Residues: 1-289; Coverage: 96%)
Gene names: DOHH, HLRC1
Sequence domains: HEAT repeats

Ligands and Environments

3 bound ligands:
Ligand FE 4 x FE
Ligand PER 2 x PER
Ligand GAI 5 x GAI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P21
Unit cell:
a: 45.933Å b: 48.636Å c: 124.087Å
α: 90° β: 99.27° γ: 90°
R-values:
R R work R free
0.205 0.203 0.246
Expression system: Escherichia coli BL21

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Citations

7 review citations

Translation Factor eIF5A, Modification with Hypusine and Role in Regulation of Gene Expression. eIF5A as a Target for Pharmacological Interventions.
Turpaev KT. (2018)
6 more

5 mentions without citation

Dioxygen Activation by Nonheme Diiron Enzymes: Diverse Dioxygen Adducts, High-Valent Intermediates, and Related Model Complexes.
Jasniewski et al. (2018)
4 more