› 4d51

X-ray diffraction
2.3Å resolution

Crystal structure of CymA from Klebsiella oxytoca

Released:
DOI: 10.2210/pdb4d51/pdb
PDB entry 4d51 coloured by chain, front view.
PDB entry 4d51 coloured by chain, side view.
PDB entry 4d51 coloured by chain, top view.
Source organism: Klebsiella oxytoca
Primary publication:
Outer-membrane translocation of bulky small molecules by passive diffusion.
van den Berg B, Prathyusha Bhamidimarri S, Dahyabhai Prajapati J, Kleinekathöfer U, Winterhalter M
Proc Natl Acad Sci U S A 112 E2991-9 (2015)
PMID: 26015567

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175346 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CymA protein Chain: A
Molecule details ›
Chain: A
Length: 324 amino acids
Theoretical weight: 38.01 KDa
Source organism: Klebsiella oxytoca
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q48391 (Residues: 23-346; Coverage: 100%)
Gene name: cymA
Sequence domains: Putative cyclodextrin porin

Ligands and Environments

1 bound ligand:
Ligand C8E 1 x C8E
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: C2221
Unit cell:
a: 59.62Å b: 228.12Å c: 60.61Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.214 0.28
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Understanding antibiotic resistance via outer membrane permeability.
Ghai et al. (2018)
2 more

3 mentions without citation

Outer-membrane translocation of bulky small molecules by passive diffusion.
van den Berg et al. (2015)
2 more