› 4d52

X-ray diffraction
1.76Å resolution

CRYSTAL STRUCTURE OF FUCOSE BINDING LECTIN FROM ASPERGILLUS FUMIGATUS (AFL) IN COMPLEX WITH L-GALACTOPYRANOSE.

Released:
DOI: 10.2210/pdb4d52/pdb
PDB entry 4d52 coloured by chain, front view.
PDB entry 4d52 coloured by chain, side view.
PDB entry 4d52 coloured by chain, top view.
Source organism: Aspergillus fumigatus
Primary publication:
Structural insights into Aspergillus fumigatus lectin specificity: AFL binding sites are functionally non-equivalent.
Houser J, Komarek J, Cioci G, Varrot A, Imberty A, Wimmerova M
Acta Crystallogr D Biol Crystallogr 71 442-53 (2015)
PMID: 25760594

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175845 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fucose-specific lectin Chain: A
Molecule details ›
Chain: A
Length: 315 amino acids
Theoretical weight: 34.61 KDa
Source organism: Aspergillus fumigatus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4WW81 (Residues: 1-315; Coverage: 100%)
Gene names: AFUA_5G14740, fleA
Sequence domains: Fungal fucose-specific lectin
Structure domains: Fucose-specific lectin
Fucose-specific lectin Chains: B, D
Molecule details ›
Chains: B, D
Length: 315 amino acids
Theoretical weight: 34.64 KDa
Source organism: Aspergillus fumigatus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4WW81 (Residues: 1-315; Coverage: 100%)
Gene names: AFUA_5G14740, fleA
Sequence domains: Fungal fucose-specific lectin
Structure domains: Fucose-specific lectin
Fucose-specific lectin Chain: C
Molecule details ›
Chain: C
Length: 315 amino acids
Theoretical weight: 34.68 KDa
Source organism: Aspergillus fumigatus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4WW81 (Residues: 1-315; Coverage: 100%)
Gene names: AFUA_5G14740, fleA
Sequence domains: Fungal fucose-specific lectin
Structure domains: Fucose-specific lectin

Ligands and Environments

7 bound ligands:
Ligand GXL 14 x GXL
Ligand GIV 12 x GIV
Ligand PEG 7 x PEG
Ligand PG4 5 x PG4
Ligand ZN 1 x ZN
Ligand NA 3 x NA
Ligand PGE 3 x PGE
1 modified residue:
Ligand CSD 4 x CSD

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 80.03Å b: 70.44Å c: 117.8Å
α: 90° β: 108.34° γ: 90°
R-values:
R R work R free
0.155 0.154 0.178
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Histo-blood group antigens as mediators of infections.
Heggelund et al. (2017)
1 more

1 mention without citation

Influence of Trp flipping on carbohydrate binding in lectins. An example on Aleuria aurantia lectin AAL.
Houser et al. (2017)