4d5y

Electron Microscopy
9Å resolution

Cryo-EM structures of ribosomal 80S complexes with termination factors and cricket paralysis virus IRES reveal the IRES in the translocated state

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 46-mer (preferred)
PDBe Complex ID:
PDB-CPX-124221 (preferred)
Entry contents:
43 distinct polypeptide molecules
3 distinct RNA molecules
Macromolecules (46 distinct):
Large ribosomal subunit protein uL2 Chain: A
Molecule details ›
Chain: A
Length: 257 amino acids
Theoretical weight: 28.09 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TT27 (Residues: 1-217; Coverage: 85%)
Gene name: RPL8
Sequence domains:
60S RIBOSOMAL PROTEIN UL3 Chain: B
Molecule details ›
Chain: B
Length: 403 amino acids
Theoretical weight: 46.21 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN UL4 Chain: C
Molecule details ›
Chain: C
Length: 427 amino acids
Theoretical weight: 47.8 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN UL18 Chain: D
Molecule details ›
Chain: D
Length: 297 amino acids
Theoretical weight: 34.43 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN EL6 Chain: E
Molecule details ›
Chain: E
Length: 288 amino acids
Theoretical weight: 32.81 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN UL30 Chain: F
Molecule details ›
Chain: F
Length: 248 amino acids
Theoretical weight: 29.29 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN EL8 Chain: G
Molecule details ›
Chain: G
Length: 266 amino acids
Theoretical weight: 30.06 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN UL6 Chain: H
Molecule details ›
Chain: H
Length: 192 amino acids
Theoretical weight: 21.9 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN UL16 Chain: I
Molecule details ›
Chain: I
Length: 214 amino acids
Theoretical weight: 24.66 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN UL5 Chain: J
Molecule details ›
Chain: J
Length: 178 amino acids
Theoretical weight: 20.29 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN EL13 Chain: L
Molecule details ›
Chain: L
Length: 211 amino acids
Theoretical weight: 24.32 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN EL14 Chain: M
Molecule details ›
Chain: M
Length: 215 amino acids
Theoretical weight: 23.49 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN EL15 Chain: N
Molecule details ›
Chain: N
Length: 204 amino acids
Theoretical weight: 24.21 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN UL13 Chain: O
Molecule details ›
Chain: O
Length: 203 amino acids
Theoretical weight: 23.63 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN UL22 Chain: P
Molecule details ›
Chain: P
Length: 184 amino acids
Theoretical weight: 21.44 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN EL18 Chain: Q
Molecule details ›
Chain: Q
Length: 188 amino acids
Theoretical weight: 21.69 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN UL19 Chain: R
Molecule details ›
Chain: R
Length: 196 amino acids
Theoretical weight: 23.54 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN EL20 Chain: S
Molecule details ›
Chain: S
Length: 176 amino acids
Theoretical weight: 20.81 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN EL21 Chain: T
Molecule details ›
Chain: T
Length: 160 amino acids
Theoretical weight: 18.61 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN EL22 Chain: U
Molecule details ›
Chain: U
Length: 128 amino acids
Theoretical weight: 14.81 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN UL14 Chain: V
Molecule details ›
Chain: V
Length: 140 amino acids
Theoretical weight: 14.89 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN EL24 Chain: W
Molecule details ›
Chain: W
Length: 157 amino acids
Theoretical weight: 17.83 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN UL23 Chain: X
Molecule details ›
Chain: X
Length: 156 amino acids
Theoretical weight: 17.74 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN UL24 Chain: Y
Molecule details ›
Chain: Y
Length: 145 amino acids
Theoretical weight: 17.3 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN EL27 Chain: Z
Molecule details ›
Chain: Z
Length: 136 amino acids
Theoretical weight: 15.84 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN UL15 Chain: a
Molecule details ›
Chain: a
Length: 148 amino acids
Theoretical weight: 16.6 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN EL29 Chain: b
Molecule details ›
Chain: b
Length: 159 amino acids
Theoretical weight: 17.8 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN EL30 Chain: c
Molecule details ›
Chain: c
Length: 115 amino acids
Theoretical weight: 12.81 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN EL31 Chain: d
Molecule details ›
Chain: d
Length: 125 amino acids
Theoretical weight: 14.49 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN EL32 Chain: e
Molecule details ›
Chain: e
Length: 135 amino acids
Theoretical weight: 15.9 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN EL33 Chain: f
Molecule details ›
Chain: f
Length: 110 amino acids
Theoretical weight: 12.56 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN EL34 Chain: g
Molecule details ›
Chain: g
Length: 117 amino acids
Theoretical weight: 13.33 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN UL29 Chain: h
Molecule details ›
Chain: h
Length: 123 amino acids
Theoretical weight: 14.59 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN EL36 Chain: i
Molecule details ›
Chain: i
Length: 105 amino acids
Theoretical weight: 12.29 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN EL37 Chain: j
Molecule details ›
Chain: j
Length: 97 amino acids
Theoretical weight: 11.11 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN EL38 Chain: k
Molecule details ›
Chain: k
Length: 70 amino acids
Theoretical weight: 8.24 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN EL39 Chain: l
Molecule details ›
Chain: l
Length: 51 amino acids
Theoretical weight: 6.43 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN EL40 Chain: m
Molecule details ›
Chain: m
Length: 128 amino acids
Theoretical weight: 14.76 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN EL41 Chain: n
Molecule details ›
Chain: n
Length: 25 amino acids
Theoretical weight: 3.47 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN EL44 Chain: o
Molecule details ›
Chain: o
Length: 106 amino acids
Theoretical weight: 12.48 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN EL43 Chain: p
Molecule details ›
Chain: p
Length: 92 amino acids
Theoretical weight: 10.3 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN EL28 Chain: t
Molecule details ›
Chain: t
Length: 137 amino acids
Theoretical weight: 15.78 KDa
Source organism: Oryctolagus cuniculus
60S RIBOSOMAL PROTEIN UL1 Chain: u
Molecule details ›
Chain: u
Length: 210 amino acids
Theoretical weight: 23.17 KDa
Source organism: Oryctolagus cuniculus
28S ribosomal RNA Chain: 2
Molecule details ›
Chain: 2
Length: 5025 nucleotides
Theoretical weight: 1625.92 KDa
Sequence domains: Eukaryotic large subunit ribosomal RNA
5.8S ribosomal RNA Chain: 3
Molecule details ›
Chain: 3
Length: 194 nucleotides
Theoretical weight: 62.62 KDa
Sequence domains: 5.8S ribosomal RNA
5S ribosomal RNA Chain: 4
Molecule details ›
Chain: 4
Length: 119 nucleotides
Theoretical weight: 38.39 KDa
Sequence domains: 5S ribosomal RNA

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution:
Relevant EMDB volumes: EMD-2810