› 4d7m

X-ray diffraction
1.55Å resolution

TetR(D) in complex with anhydrotetracycline and magnesium

Released:
DOI: 10.2210/pdb4d7m/pdb
PDB entry 4d7m coloured by chain, front view.
PDB entry 4d7m coloured by chain, side view.
PDB entry 4d7m coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Tetracycline repressor allostery does not depend on divalent metal recognition.
Werten S, Dalm D, Palm GJ, Grimm CC, Hinrichs W
Biochemistry 53 7990-8 (2014)
PMID: 25432019

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142298 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tetracycline repressor protein class D Chain: A
Molecule details ›
Chain: A
Length: 217 amino acids
Theoretical weight: 24.33 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P0ACT4 (Residues: 3-218; Coverage: 99%)
Gene name: tetR
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand TDC 1 x TDC
Ligand MG 1 x MG
Ligand SO4 1 x SO4
Ligand CL 4 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X13
Spacegroup: I4122
Unit cell:
a: 65.628Å b: 65.628Å c: 178.598Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.176 0.217
Expression system: Escherichia coli K-12

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Citations

6 citation in other articles

Modular organisation of inducer recognition and allostery in the tetracycline repressor.
Werten et al. (2016)
5 more