› 4d81

X-ray diffraction
2.4Å resolution

Metallosphera sedula Vps4 crystal structure

Released:
DOI: 10.2210/pdb4d81/pdb
PDB entry 4d81 coloured by chain, front view.
PDB entry 4d81 coloured by chain, side view.
PDB entry 4d81 coloured by chain, top view.
Source organism: Metallosphaera sedula
Primary publication:
Asymmetric ring structure of Vps4 required for ESCRT-III disassembly.
Caillat C, Macheboeuf P, Wu Y, McCarthy AA, Boeri-Erba E, Effantin G, Göttlinger HG, Weissenhorn W, Renesto P
Nat Commun 6 8781 (2015)
PMID: 26632262

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107504 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AAA ATPase, central domain protein Chain: A
Molecule details ›
Chain: A
Length: 283 amino acids
Theoretical weight: 32.23 KDa
Source organism: Metallosphaera sedula
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A4YHC5 (Residues: 88-369; Coverage: 76%)
Gene name: Msed_1672
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P65
Unit cell:
a: 65.269Å b: 65.269Å c: 112.521Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.21 0.207 0.263
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Reverse-topology membrane scission by the ESCRT proteins.
Schöneberg et al. (2017)
8 more

4 mentions without citation

Fundamental Characteristics of AAA+ Protein Family Structure and Function.
Miller et al. (2016)
3 more