› 4d8h

X-ray diffraction
1.9Å resolution

Crystal structure of Symfoil-4P/PV2: de novo designed beta-trefoil architecture with symmetric primary structure, primitive version 2 (6xLeu / PV1)

Released:
DOI: 10.2210/pdb4d8h/pdb
PDB entry 4d8h coloured by chain, front view.
PDB entry 4d8h coloured by chain, side view.
PDB entry 4d8h coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Simplified protein design biased for prebiotic amino acids yields a foldable, halophilic protein.
Longo LM, Lee J, Blaber M
Proc Natl Acad Sci U S A 110 2135-9 (2013)
PMID: 23341608

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164384 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
de novo protein Chain: A
Molecule details ›
Chain: A
Length: 132 amino acids
Theoretical weight: 14.32 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Structure domains: Trefoil (Acidic Fibroblast Growth Factor, subunit A)

Ligands and Environments

2 bound ligands:
Ligand TRS 1 x TRS
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 46.584Å b: 48.696Å c: 64.876Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.165 0.214
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Life at high salt concentrations, intracellular KCl concentrations, and acidic proteomes.
Oren A. (2013)
11 more

2 mentions without citation

Simplified protein design biased for prebiotic amino acids yields a foldable, halophilic protein.
Longo et al. (2013)
1 more