› 4d8m

X-ray diffraction
2.3Å resolution

Crystal structure of Bacillus thuringiensis Cry5B nematocidal toxin

Released:
DOI: 10.2210/pdb4d8m/pdb
PDB entry 4d8m coloured by chain, front view.
PDB entry 4d8m coloured by chain, side view.
PDB entry 4d8m coloured by chain, top view.
Source organism: Bacillus thuringiensis
Primary publication:
Structure and Glycolipid Binding Properties of the Nematicidal Protein Cry5B.
Hui F, Scheib U, Hu Y, Sommer RJ, Aroian RV, Ghosh P
Biochemistry (2012)
PMID: 23150986

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175072 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pesticidal crystal protein Cry5Ba Chain: A
Molecule details ›
Chain: A
Length: 587 amino acids
Theoretical weight: 66.09 KDa
Source organism: Bacillus thuringiensis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q45712 (Residues: 112-698; Coverage: 47%)
Gene names: cry5Ba, cryVB(a)
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P63
Unit cell:
a: 115.528Å b: 115.528Å c: 110.328Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.232 0.173 0.218
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Molecular approaches to improve the insecticidal activity of Bacillus thuringiensis Cry toxins.
Lucena et al. (2014)
4 more

9 mentions without citation

Structural insights into Bacillus thuringiensis Cry, Cyt and parasporin toxins.
Xu et al. (2014)
8 more