› 4dam

X-ray diffraction
1.7Å resolution

Crystal structure of small single-stranded DNA-binding protein from Streptomyces coelicolor

Released:
DOI: 10.2210/pdb4dam/pdb
PDB entry 4dam coloured by chain, front view.
PDB entry 4dam coloured by chain, side view.
PDB entry 4dam coloured by chain, top view.
Source organism: Streptomyces coelicolor
Primary publication:
Structure-function relationships of two paralogous single-stranded DNA-binding proteins from Streptomyces coelicolor: implication of SsbB in chromosome segregation during sporulation.
Paradzik T, Ivic N, Filic Z, Manjasetty BA, Herron P, Luic M, Vujaklija D
Nucleic Acids Res 41 3659-72 (2013)
PMID: 23393191

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192173 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Single-stranded DNA-binding protein 1 Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 128 amino acids
Theoretical weight: 14.12 KDa
Source organism: Streptomyces coelicolor
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KYI9 (Residues: 1-116; Coverage: 74%)
Gene names: SCC61A.04c, SCO2683, ssb1
Sequence domains: Single-strand binding protein family
Structure domains: Nucleic acid-binding proteins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P32
Unit cell:
a: 150.004Å b: 150.004Å c: 54.7Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.155 0.153 0.192
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Compaction and control-the role of chromosome-organizing proteins in Streptomyces.
Szafran et al. (2020)
11 other articles

1 mention without citation

Structure-function relationships of two paralogous single-stranded DNA-binding proteins from Streptomyces coelicolor: implication of SsbB in chromosome segregation during sporulation.
Paradzik et al. (2013)