› 4dct

X-ray diffraction
2.3Å resolution

Crystal Structure of B. subtilis EngA in complex with half-occupacy GDP

Released:
DOI: 10.2210/pdb4dct/pdb
PDB entry 4dct coloured by chain, front view.
PDB entry 4dct coloured by chain, side view.
PDB entry 4dct coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Potassium acts as a GTPase-activating element on each nucleotide-binding domain of the essential Bacillus subtilis EngA.
Foucher AE, Reiser JB, Ebel C, Housset D, Jault JM
PLoS One 7 e46795 (2012)
PMID: 23056455

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156259 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTPase Der Chain: A
Molecule details ›
Chain: A
Length: 456 amino acids
Theoretical weight: 51 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P50743 (Residues: 1-436; Coverage: 100%)
Gene names: BSU22840, der, engA, yphC
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GDP 2 x GDP
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 62.76Å b: 65.26Å c: 112.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.224 0.295
Expression system: Escherichia coli

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Citations

3 review citations

Epithelial sodium transport and its control by aldosterone: the story of our internal environment revisited.
Rossier et al. (2015)
2 more

79 mentions without citation

Motion management in gastrointestinal cancers.
Abbas et al. (2014)
78 more