› 4ddq

X-ray diffraction
3.3Å resolution

Structural plasticity of the Bacillus subtilis GyrA homodimer

Released:
DOI: 10.2210/pdb4ddq/pdb
PDB entry 4ddq coloured by chain, front view.
PDB entry 4ddq coloured by chain, side view.
PDB entry 4ddq coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Mapping the spectrum of conformational states of the DNA- and C-gates in Bacillus subtilis gyrase.
Rudolph MG, Klostermeier D
J Mol Biol 425 2632-40 (2013)
PMID: 23602808

Function and Biology Details

Reaction catalysed: 
ATP-dependent breakage, passage and rejoining of double-stranded DNA
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138727 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA gyrase subunit A Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 502 amino acids
Theoretical weight: 56.75 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P05653 (Residues: 1-502; Coverage: 61%)
Gene names: BSU00070, cafB, gyrA, nalA
Sequence domains: DNA gyrase/topoisomerase IV, subunit A
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand K 6 x K
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 152.803Å b: 165.106Å c: 180.582Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.174 0.235
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Fluoroquinolone resistance: mechanisms, impact on bacteria, and role in evolutionary success.
Redgrave et al. (2014)
4 more

5 mentions without citation

The Blueprint of a Minimal Cell: MiniBacillus.
Reuß et al. (2016)
4 more