› 4ddu

X-ray diffraction
3Å resolution

Thermotoga maritima reverse gyrase, C2 FORM 1

Released:
DOI: 10.2210/pdb4ddu/pdb
PDB entry 4ddu coloured by chain, front view.
PDB entry 4ddu coloured by chain, side view.
PDB entry 4ddu coloured by chain, top view.
Source organism: Thermotoga maritima
Primary publication:
Crystal structures of Thermotoga maritima reverse gyrase: inferences for the mechanism of positive DNA supercoiling.
Rudolph MG, del Toro Duany Y, Jungblut SP, Ganguly A, Klostermeier D
Nucleic Acids Res 41 1058-70 (2013)
PMID: 23209025

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129368 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Reverse gyrase Chain: A
Molecule details ›
Chain: A
Length: 1104 amino acids
Theoretical weight: 128.48 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli
UniProt:
  • Canonical: O51934 (Residues: 1-1104; Coverage: 100%)
Gene names: TM_0173, rgy, topR
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 3 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: C2
Unit cell:
a: 169.961Å b: 73.912Å c: 111.371Å
α: 90° β: 116.59° γ: 90°
R-values:
R R work R free
0.218 0.214 0.278
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Genome stability: recent insights in the topoisomerase reverse gyrase and thermophilic DNA alkyltransferase.
Vettone et al. (2014)
2 more

9 mentions without citation

DNA topoisomerases: Advances in understanding of cellular roles and multi-protein complexes via structure-function analysis.
McKie et al. (2021)
8 more