› 4ddw

X-ray diffraction
3.9Å resolution

Thermotoga maritima reverse gyrase, c-centered orthorhombic form

Released:
DOI: 10.2210/pdb4ddw/pdb
PDB entry 4ddw coloured by chain, front view.
PDB entry 4ddw coloured by chain, side view.
PDB entry 4ddw coloured by chain, top view.
Source organism: Thermotoga maritima
Primary publication:
Crystal structures of Thermotoga maritima reverse gyrase: inferences for the mechanism of positive DNA supercoiling.
Rudolph MG, del Toro Duany Y, Jungblut SP, Ganguly A, Klostermeier D
Nucleic Acids Res 41 1058-70 (2013)
PMID: 23209025

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129368 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Reverse gyrase Chain: A
Molecule details ›
Chain: A
Length: 1104 amino acids
Theoretical weight: 128.48 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli
UniProt:
  • Canonical: O51934 (Residues: 1-1104; Coverage: 100%)
Gene names: TM_0173, rgy, topR
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand POP 1 x POP
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: C2221
Unit cell:
a: 174.325Å b: 175.646Å c: 104.135Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.209 0.289
Expression system: Escherichia coli

Quick links

Citations

8 review citations

DNA topoisomerases: Advances in understanding of cellular roles and multi-protein complexes via structure-function analysis.
McKie et al. (2021)
7 more

1 mention without citation

Crystal structures of Thermotoga maritima reverse gyrase: inferences for the mechanism of positive DNA supercoiling.
Rudolph et al. (2013)