› 4df3

X-ray diffraction
1.73Å resolution

Crystal Structure of Aeropyrum pernix fibrillarin in complex with natively bound S-adenosyl-L-methionine at 1.7A

Released:
DOI: 10.2210/pdb4df3/pdb
PDB entry 4df3 coloured by chain, front view.
PDB entry 4df3 coloured by chain, side view.
PDB entry 4df3 coloured by chain, top view.
Source organism: Aeropyrum pernix
Primary publication:
Structure of Aeropyrum pernix fibrillarin in complex with natively bound S-adenosyl-L-methionine at 1.7 Å resolution.
de Silva U, Zhou Z, Brown BA
Acta Crystallogr Sect F Struct Biol Cryst Commun 68 854-9 (2012)
PMID: 22869109

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195433 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 233 amino acids
Theoretical weight: 26.7 KDa
Source organism: Aeropyrum pernix
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y9U3 (Residues: 1-233; Coverage: 100%)
Gene names: APE_2196, flpA
Sequence domains: Fibrillarin
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SAM 2 x SAM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P1
Unit cell:
a: 39.639Å b: 52.087Å c: 63.03Å
α: 66.28° β: 84.55° γ: 77.61°
R-values:
R R work R free
0.19 0.187 0.234
Expression system: Escherichia coli

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Citations

1 review citation

Fibrillarin from Archaea to human.
Rodriguez-Corona et al. (2015)

2 mentions without citation

Structure of Aeropyrum pernix fibrillarin in complex with natively bound S-adenosyl-L-methionine at 1.7 Å resolution.
de Silva et al. (2012)
1 more