› 4dfl

X-ray diffraction
1.98Å resolution

Crystal structure of spleen tyrosine kinase complexed with a sulfonamidopyrazine piperidine inhibitor

Released:
DOI: 10.2210/pdb4dfl/pdb
PDB entry 4dfl coloured by chain, front view.
PDB entry 4dfl coloured by chain, side view.
PDB entry 4dfl coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Pyrazine-based Syk kinase inhibitors.
Forns P, Esteve C, Taboada L, Alonso JA, Orellana A, Maldonado M, Carreño C, Ramis I, López M, Miralpeix M, Vidal B
Bioorg Med Chem Lett 22 2784-8 (2012)
PMID: 22425453

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155056 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein kinase SYK Chain: A
Molecule details ›
Chain: A
Length: 274 amino acids
Theoretical weight: 31.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P43405 (Residues: 363-635; Coverage: 43%)
Gene name: SYK
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand 0K0 1 x 0K0
Ligand EDO 1 x EDO
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 40.028Å b: 85.004Å c: 90.748Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.196 0.243
Expression system: Escherichia coli

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Citations

8 citation in other articles

Syk inhibitors with high potency in presence of blood.
Thoma et al. (2014)
7 more