› 4dgk

X-ray diffraction
2.35Å resolution

Crystal structure of Phytoene desaturase CRTI from Pantoea ananatis

Released:
DOI: 10.2210/pdb4dgk/pdb
PDB entry 4dgk coloured by chain, front view.
PDB entry 4dgk coloured by chain, side view.
PDB entry 4dgk coloured by chain, top view.
Source organism: Pantoea ananatis
Primary publication:
On the structure and function of the phytoene desaturase CRTI from Pantoea ananatis, a membrane-peripheral and FAD-dependent oxidase/isomerase.
Schaub P, Yu Q, Gemmecker S, Poussin-Courmontagne P, Mailliot J, McEwen AG, Ghisla S, Al-Babili S, Cavarelli J, Beyer P
PLoS One 7 e39550 (2012)
PMID: 22745782

Function and Biology Details

Reaction catalysed: 
(1a) 15-cis-phytoene + acceptor = all-trans-phytofluene + reduced acceptor
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149359 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phytoene desaturase (lycopene-forming) Chain: A
Molecule details ›
Chain: A
Length: 501 amino acids
Theoretical weight: 56.1 KDa
Source organism: Pantoea ananatis
Expression system: Escherichia coli
UniProt:
  • Canonical: P21685 (Residues: 1-492; Coverage: 100%)
Gene name: crtI
Sequence domains: Flavin containing amine oxidoreductase
Structure domains: FAD/NAD(P)-binding domain

Ligands and Environments

2 bound ligands:
Ligand EDO 5 x EDO
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P3221
Unit cell:
a: 90.69Å b: 90.69Å c: 130.03Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.192 0.19 0.23
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Plant carotenoids: genomics meets multi-gene engineering.
Giuliano G. (2014)
4 more

8 mentions without citation

Mechanistic aspects of carotenoid biosynthesis.
Moise et al. (2014)
7 more