› 4dhd

X-ray diffraction
1.65Å resolution

Crystal structure of isoprenoid synthase A3MSH1 (TARGET EFI-501992) from Pyrobaculum calidifontis

Released:
DOI: 10.2210/pdb4dhd/pdb
PDB entry 4dhd coloured by chain, front view.
PDB entry 4dhd coloured by chain, side view.
PDB entry 4dhd coloured by chain, top view.
Source organism: Pyrobaculum calidifontis JCM 11548
Primary publication:
Prediction of function for the polyprenyl transferase subgroup in the isoprenoid synthase superfamily.
Wallrapp FH, Pan JJ, Ramamoorthy G, Almonacid DE, Hillerich BS, Seidel R, Patskovsky Y, Babbitt PC, Almo SC, Jacobson MP, Poulter CD
Proc Natl Acad Sci U S A 110 E1196-202 (2013)
PMID: 23493556

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107175 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polyprenyl synthetase Chain: A
Molecule details ›
Chain: A
Length: 358 amino acids
Theoretical weight: 40.15 KDa
Source organism: Pyrobaculum calidifontis JCM 11548
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A3MSH1 (Residues: 1-334; Coverage: 100%)
Gene name: Pcal_0150
Sequence domains: Polyprenyl synthetase
Structure domains: Farnesyl Diphosphate Synthase

Ligands and Environments

3 bound ligands:
Ligand PO4 1 x PO4
Ligand CL 1 x CL
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2221
Unit cell:
a: 61.401Å b: 89.269Å c: 149.542Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.181 0.213
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

15 review citations

Enzyme Function Initiative-Enzyme Similarity Tool (EFI-EST): A web tool for generating protein sequence similarity networks.
Gerlt et al. (2015)
14 more

1 mention without citation

Prediction of function for the polyprenyl transferase subgroup in the isoprenoid synthase superfamily.
Wallrapp et al. (2013)