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› 4dhk

X-ray diffraction
2.05Å resolution

Crystal structure of a deoxycytidine triphosphate deaminase (dCTP deaminase) from Burkholderia thailandensis

Released:
DOI: 10.2210/pdb4dhk/pdb
Model geometry
Fit model/data
PDB entry 4dhk coloured by chain, front view.
PDB entry 4dhk coloured by chain, side view.
PDB entry 4dhk coloured by chain, top view.
Source organism: Burkholderia thailandensis E264
Primary publication:
Combining functional and structural genomics to sample the essential Burkholderia structome.
Baugh L, Gallagher LA, Patrapuvich R, Clifton MC, Gardberg AS, Edwards TE, Armour B, Begley DW, Dieterich SH, Dranow DM, Abendroth J, Fairman JW, Fox D, Staker BL, Phan I, Gillespie A, Choi R, Nakazawa-Hewitt S, Nguyen MT, Napuli A, Barrett L, Buchko GW, Stacy R, Myler PJ, Stewart LJ, Manoil C, Van Voorhis WC
OpenAccess logo PLoS One 8 e53851 (2013)
PMID: 23382856

Function and Biology Details

Reaction catalysed: 
dCTP + H2O + H(+) = dUTP + NH4(+).
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo hexamer
homo trimer
Assembly name:
PDBe Complex ID:
PDB-CPX-173988 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
dCTP deaminase Chains: A, B
Molecule details ›
Chains: A, B
Length: 191 amino acids
Theoretical weight: 21.51 KDa
Source organism: Burkholderia thailandensis E264
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q2T083 (Residues: 1-189; Coverage: 100%)
Gene names: BTH_I0860, dcd
Sequence domains: dCTP deaminase-like
Structure domains: Deoxyuridine 5'-Triphosphate Nucleotidohydrolase; Chain A

Ligands and Environments

1 bound ligand:
Ligand EDO 7 x EDO
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: R3
Unit cell:
a: 92.87Å b: 92.87Å c: 137.59Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.168 0.168 0.186
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

Prokaryotic Heme Biosynthesis: Multiple Pathways to a Common Essential Product.
Dailey et al. (2017)
10 more

3 mentions without citation

Combining functional and structural genomics to sample the essential Burkholderia structome.
Baugh et al. (2013)
2 more