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› 4die

X-ray diffraction
2.65Å resolution

Crystal structure of a cytidylate kinase CmK from Mycobacterium abscessus bound to cytidine-5'-monophosphate

Released:
DOI: 10.2210/pdb4die/pdb
Model geometry
Fit model/data
PDB entry 4die coloured by chain, front view.
PDB entry 4die coloured by chain, side view.
PDB entry 4die coloured by chain, top view.
Source organism: Mycobacteroides abscessus ATCC 19977
Primary publication:
Increasing the structural coverage of tuberculosis drug targets.
Baugh L, Phan I, Begley DW, Clifton MC, Armour B, Dranow DM, Taylor BM, Muruthi MM, Abendroth J, Fairman JW, Fox D, Dieterich SH, Staker BL, Gardberg AS, Choi R, Hewitt SN, Napuli AJ, Myers J, Barrett LK, Zhang Y, Ferrell M, Mundt E, Thompkins K, Tran N, Lyons-Abbott S, Abramov A, Sekar A, Serbzhinskiy D, Lorimer D, Buchko GW, Stacy R, Stewart LJ, Edwards TE, Van Voorhis WC, Myler PJ
Tuberculosis (Edinb) 95 142-8 (2015)
PMID: 25613812

Function and Biology Details

Reaction catalysed: 
(1) CMP + ATP = CDP + ADP.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109331 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytidylate kinase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 228 amino acids
Theoretical weight: 23.87 KDa
Source organism: Mycobacteroides abscessus ATCC 19977
Expression system: Escherichia coli
UniProt:
  • Canonical: B1MB31 (Residues: 2-223; Coverage: 100%)
Gene names: MAB_2371, cmk
Sequence domains: Cytidylate kinase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand C5P 4 x C5P
Ligand PO4 4 x PO4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: P21
Unit cell:
a: 70.76Å b: 96.52Å c: 78.79Å
α: 90° β: 97.22° γ: 90°
R-values:
R R work R free
0.233 0.231 0.27
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Pleiotropic Functions of Tumor Suppressor WWOX in Normal and Cancer Cells.
Abu-Remaileh et al. (2015)
5 more

1 mention without citation

Increasing the structural coverage of tuberculosis drug targets.
Baugh et al. (2015)