› 4dif

X-ray diffraction
1.52Å resolution

Structure of A1-type ketoreductase

Released:
DOI: 10.2210/pdb4dif/pdb
PDB entry 4dif coloured by chain, front view.
PDB entry 4dif coloured by chain, side view.
PDB entry 4dif coloured by chain, top view.
Source organism: Streptomyces nodosus
Entry authors: Zheng J, Keatinge-Clay AT

Function and Biology Details

Reaction catalysed: 
(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP(+) = 3-oxoacyl-[acyl-carrier-protein] + NADPH
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188105 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AmphB Chains: A, B
Molecule details ›
Chains: A, B
Length: 496 amino acids
Theoretical weight: 51.48 KDa
Source organism: Streptomyces nodosus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q93NW7 (Residues: 2529-3003; Coverage: 15%)
Gene name: amphB
Sequence domains: KR domain
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NDP 2 x NDP
1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P1
Unit cell:
a: 61.546Å b: 63.716Å c: 71.983Å
α: 72.92° β: 67.22° γ: 89.74°
R-values:
R R work R free
0.2 0.198 0.224
Expression system: Escherichia coli

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