› 4dio

X-ray diffraction
2.6Å resolution

The crystal structure of transhydrogenase from Sinorhizobium meliloti

Released:
DOI: 10.2210/pdb4dio/pdb
PDB entry 4dio coloured by chain, front view.
PDB entry 4dio coloured by chain, side view.
PDB entry 4dio coloured by chain, top view.
Source organism: Sinorhizobium meliloti SM11
Entry authors: Zhang Z, Chamala S, Evans B, Foti R, Gizzi A, Hillerich B, Kar A, Lafleur J, Seidel R, Villigas G, Zencheck W, Almo SC, Swaminathan S, New York Structural Genomics Research Consortium (NYSGRC)

Function and Biology Details

Reaction catalysed: 
NADPH + NAD(+) + H(+)(Side 1) = NADP(+) + H(+)(Side 2) + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123684 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
proton-translocating NAD(P)(+) transhydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 405 amino acids
Theoretical weight: 43.19 KDa
Source organism: Sinorhizobium meliloti SM11
Expression system: Escherichia coli
UniProt:
  • Canonical: F7X914 (Residues: 30-411; Coverage: 93%)
Gene names: SM11_chr3030, pntAa
Sequence domains:
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 34 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 65.777Å b: 69.67Å c: 138.387Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.235 0.232 0.287
Expression system: Escherichia coli

Quick links