› 4dkf

X-ray diffraction
2.61Å resolution

Crystal Structure of Human Interleukin-34 Bound to FAb2

Released:
DOI: 10.2210/pdb4dkf/pdb
PDB entry 4dkf coloured by chain, front view.
PDB entry 4dkf coloured by chain, side view.
PDB entry 4dkf coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for the dual recognition of helical cytokines IL-34 and CSF-1 by CSF-1R.
Ma X, Lin WY, Chen Y, Stawicki S, Mukhyala K, Wu Y, Martin F, Bazan JF, Starovasnik MA
Structure 20 676-87 (2012)
PMID: 22483114

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero hexamer (preferred)
hetero dodecamer
Assembly name:
PDBe Complex ID:
PDB-CPX-212424 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
Interleukin-34 Chains: A, B
Molecule details ›
Chains: A, B
Length: 190 amino acids
Theoretical weight: 21.86 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q6ZMJ4 (Residues: 21-193; Coverage: 78%)
Gene names: C16orf77, IL34
Sequence domains: Interleukin 34
Structure domains: Interleukin-34
FAb2 Heavy Chain Chains: H, I
Molecule details ›
Chains: H, I
Length: 226 amino acids
Theoretical weight: 23.76 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins
FAb2 Light Chain Chains: L, M
Molecule details ›
Chains: L, M
Length: 214 amino acids
Theoretical weight: 23.34 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, MAN, BMA
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: C2
Unit cell:
a: 117.917Å b: 181.822Å c: 79.254Å
α: 90° β: 118.27° γ: 90°
R-values:
R R work R free
0.227 0.225 0.256
Expression systems:
  • Trichoplusia ni
  • Cricetulus griseus

Quick links

Citations

39 review citations

From Monocytes to M1/M2 Macrophages: Phenotypical vs. Functional Differentiation.
Italiani et al. (2014)
38 more

4 mentions without citation

Challenging the state of the art in protein structure prediction: Highlights of experimental target structures for the 10th Critical Assessment of Techniques for Protein Structure Prediction Experiment CASP10.
Kryshtafovych et al. (2014)
3 more