› 4dnb

X-ray diffraction
2Å resolution

METHYLATION OF THE ECORI RECOGNITION SITE DOES NOT ALTER DNA CONFORMATION. THE CRYSTAL STRUCTURE OF D(CGCGAM6ATTCGCG) AT 2.0 ANGSTROMS RESOLUTION

Released:
DOI: 10.2210/pdb4dnb/pdb
PDB entry 4dnb coloured by chain, front view.
PDB entry 4dnb coloured by chain, side view.
PDB entry 4dnb coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
Methylation of the EcoRI recognition site does not alter DNA conformation: the crystal structure of d(CGCGAm6ATTCGCG) at 2.0-A resolution.
Frederick CA, Quigley GJ, van der Marel GA, van Boom JH, Wang AH, Rich A
J Biol Chem 263 17872-9 (1988)
PMID: 2846582

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117332 (preferred)
Entry contents:
1 distinct DNA molecule
Macromolecule:
DNA (5'-D(*CP*GP*CP*GP*AP*(6MA)P*TP*TP*CP*GP*CP*G)-3') Chains: A, B
Molecule details ›
Chains: A, B
Length: 12 nucleotides
Theoretical weight: 3.68 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand 6MA 2 x 6MA

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 25.62Å b: 40.39Å c: 67.32Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 not available not available
Expression system: Not provided

Quick links

Citations

9 review citations

DNA methylation. The effect of minor bases on DNA-protein interactions.
Adams RL. (1990)
8 more