› 4dnq

X-ray diffraction
2.8Å resolution

Crystal Structure of DAD2 S96A mutant

Released:
DOI: 10.2210/pdb4dnq/pdb
PDB entry 4dnq coloured by chain, front view.
PDB entry 4dnq coloured by chain, side view.
PDB entry 4dnq coloured by chain, top view.
Source organism: Petunia x hybrida
Primary publication:
DAD2 is an α/β hydrolase likely to be involved in the perception of the plant branching hormone, strigolactone.
Hamiaux C, Drummond RS, Janssen BJ, Ledger SE, Cooney JM, Newcomb RD, Snowden KC
Curr Biol 22 2032-6 (2012)
PMID: 22959345

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125406 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable strigolactone esterase DAD2 Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 269 amino acids
Theoretical weight: 29.85 KDa
Source organism: Petunia x hybrida
Expression system: Escherichia coli
UniProt:
  • Canonical: J9U5U9 (Residues: 1-267; Coverage: 100%)
Gene name: DAD2
Sequence domains: Alpha/beta hydrolase family
Structure domains: alpha/beta hydrolase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P31
Unit cell:
a: 176.66Å b: 176.66Å c: 107.67Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.244 0.242 0.272
Expression system: Escherichia coli

Quick links

Citations

89 review citations

Strigolactones, a novel carotenoid-derived plant hormone.
Al-Babili et al. (2015)
88 more

2 mentions without citation

Target sites for chemical regulation of strigolactone signaling.
Nakamura et al. (2014)
1 more