› 4do7

X-ray diffraction
1.7Å resolution

Crystal structure of an amidohydrolase (cog3618) from burkholderia multivorans (target efi-500235) with bound zn, space group c2

Released:
DOI: 10.2210/pdb4do7/pdb
Model geometry
Fit model/data
PDB entry 4do7 coloured by chain, front view.
PDB entry 4do7 coloured by chain, side view.
PDB entry 4do7 coloured by chain, top view.
Source organism: Burkholderia multivorans ATCC 17616
Entry authors: Vetting MW, Toro R, Bhosle R, Wasserman SR, Morisco LL, Sojitra S, Seidel RD, Hillerich B, Washington E, Scott Glenn A, Chowdhury S, Evans B, Hammonds J, Al Obaidi NF, Zencheck WD, Imker HJ, Gerlt JA, Raushel FM, Almo SC, Enzyme Function Initiative (EFI)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102062 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-fucono-1,5-lactonase Chains: A, B
Molecule details ›
Chains: A, B
Length: 303 amino acids
Theoretical weight: 33.96 KDa
Source organism: Burkholderia multivorans ATCC 17616
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H3KNC4 (Residues: 1-296; Coverage: 100%)
Gene name: BMULJ_04915
Sequence domains: Amidohydrolase
Structure domains: Metal-dependent hydrolases

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 31-ID
Spacegroup: C2
Unit cell:
a: 103.332Å b: 76.728Å c: 82.862Å
α: 90° β: 117.67° γ: 90°
R-values:
R R work R free
0.178 0.176 0.209
Expression system: Escherichia coli BL21(DE3)

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