4dr7

X-ray diffraction
3.75Å resolution

Crystal structure of the Thermus thermophilus (HB8) 30S ribosomal subunit with codon, crystallographically disordered near-cognate transfer RNA anticodon stem-loop mismatched at the second codon position, and streptomycin bound

Released:
Model geometry
Fit model/data

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 25-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-302487 (preferred)
Entry contents:
20 distinct polypeptide molecules
5 distinct RNA molecules
Macromolecules (25 distinct):
Small ribosomal subunit protein uS2 Chain: B
Molecule details ›
Chain: B
Length: 256 amino acids
Theoretical weight: 29.32 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P80371 (Residues: 1-256; Coverage: 100%)
Gene names: TTHA0861, rps2, rpsB
Sequence domains: Ribosomal protein S2
Structure domains:
Small ribosomal subunit protein uS3 Chain: C
Molecule details ›
Chain: C
Length: 239 amino acids
Theoretical weight: 26.75 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: P80372 (Residues: 1-239; Coverage: 100%)
Gene names: TTHA1686, rps3, rpsC
Sequence domains:
Structure domains:
Small ribosomal subunit protein uS4 Chain: D
Small ribosomal subunit protein uS5 Chain: E
Small ribosomal subunit protein bS6 Chain: F
Small ribosomal subunit protein uS7 Chain: G
Small ribosomal subunit protein uS8 Chain: H
Small ribosomal subunit protein uS9 Chain: I
Small ribosomal subunit protein uS10 Chain: J
Small ribosomal subunit protein uS11 Chain: K
Small ribosomal subunit protein uS12 Chain: L
Small ribosomal subunit protein uS13 Chain: M
Small ribosomal subunit protein uS14 Chain: N
Small ribosomal subunit protein uS15 Chain: O
Small ribosomal subunit protein bS16 Chain: P
Small ribosomal subunit protein uS17 Chain: Q
Small ribosomal subunit protein bS18 Chain: R
Small ribosomal subunit protein uS19 Chain: S
Small ribosomal subunit protein bS20 Chain: T
Small ribosomal subunit protein bTHX Chain: U
16S ribosomal RNA Chain: A
5'-R(*UP*UP*UP*U)-3' Chain: V
5'-R(*GP*CP*CP*UP*GP*GP*AP*AP*AP*GP*(PSU))-3' Chain: W
5'-R(P*UP*GP*GP*AP*AP*AP*GP*(PSU))-3' Chain: a
5'-R(P*UP*UP*U)-3' Chain: b

Ligands and Environments

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: NSLS BEAMLINE X25
Spacegroup: P41212
Unit cell:
a: 402.49Å b: 402.49Å c: 174.79Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.151 0.148 0.201