› 4drx

X-ray diffraction
2.22Å resolution

GTP-Tubulin in complex with a DARPIN

Released:
DOI: 10.2210/pdb4drx/pdb
PDB entry 4drx coloured by chain, front view.
PDB entry 4drx coloured by chain, side view.
PDB entry 4drx coloured by chain, top view.
Source organisms:
Primary publication:
A designed ankyrin repeat protein selected to bind to tubulin caps the microtubule plus end.
Pecqueur L, Duellberg C, Dreier B, Jiang Q, Wang C, Plückthun A, Surrey T, Gigant B, Knossow M
Proc Natl Acad Sci U S A 109 12011-6 (2012)
PMID: 22778434

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-112161 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Tubulin alpha chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 437 amino acids
Theoretical weight: 48.67 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: D0VWZ0 (Residues: 1-437; Coverage: 97%)
Sequence domains:
Structure domains:
Tubulin beta chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 431 amino acids
Theoretical weight: 48.38 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: D0VWY9 (Residues: 1-431; Coverage: 97%)
Sequence domains:
Structure domains:
Designed ankyrin repeat protein (DARPIN) D1 Chains: E, F
Molecule details ›
Chains: E, F
Length: 169 amino acids
Theoretical weight: 18.07 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Structure domains: Ankyrin repeat-containing domain

Ligands and Environments

2 bound ligands:
Ligand GTP 4 x GTP
Ligand MG 4 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P21
Unit cell:
a: 104.884Å b: 90.596Å c: 117.575Å
α: 90° β: 90.02° γ: 90°
R-values:
R R work R free
0.161 0.159 0.194
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Designed ankyrin repeat proteins (DARPins): binding proteins for research, diagnostics, and therapy.
Plückthun A. (2015)
9 more

23 mentions without citation

The contribution of αβ-tubulin curvature to microtubule dynamics.
Brouhard et al. (2014)
22 more