› 4dry

X-ray diffraction
2.5Å resolution

The crystal structure of 3-oxoacyl-[acyl-carrier-protein] reductase from Rhizobium meliloti

Released:
DOI: 10.2210/pdb4dry/pdb
PDB entry 4dry coloured by chain, front view.
PDB entry 4dry coloured by chain, side view.
PDB entry 4dry coloured by chain, top view.
Source organism: Sinorhizobium meliloti 1021
Entry authors: Zhang Z, Chamala S, Evans B, Foti R, Gizzi A, Hillerich B, Kar A, LaFleur J, Seidel R, Villigas G, Zencheck W, Almo SC, Swaminathan S, New York Structural Genomics Research Consortium (NYSGRC)

Function and Biology Details

Reaction catalysed: 
(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP(+) = 3-oxoacyl-[acyl-carrier-protein] + NADPH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187933 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-oxoacyl-[acyl-carrier-protein] reductase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 281 amino acids
Theoretical weight: 29.81 KDa
Source organism: Sinorhizobium meliloti 1021
Expression system: Escherichia coli
UniProt:
  • Canonical: Q92TX5 (Residues: 1-258; Coverage: 100%)
Gene name: SM_b20662
Sequence domains: short chain dehydrogenase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
1 modified residue:
Ligand MSE 48 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P212121
Unit cell:
a: 86.531Å b: 98.128Å c: 117.22Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.206 0.264
Expression system: Escherichia coli

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