› 4du5

X-ray diffraction
2.7Å resolution

Crystal structure of PfkB protein from Polaromonas sp. JS666

Released:
DOI: 10.2210/pdb4du5/pdb
PDB entry 4du5 coloured by chain, front view.
PDB entry 4du5 coloured by chain, side view.
PDB entry 4du5 coloured by chain, top view.
Source organism: Polaromonas sp. JS666
Entry authors: Agarwal R, Chamala S, Evans B, Foti R, Gizzi A, Hellerich B, Kar A, Lafleur J, Siedel R, Villigas G, Zencheck W, Almo SC, Swaminathan S, New York Structural Genomics Research Consortium (NYSGRC)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171473 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbohydrate kinase PfkB domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 336 amino acids
Theoretical weight: 36.04 KDa
Source organism: Polaromonas sp. JS666
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q12CR9 (Residues: 1-314; Coverage: 100%)
Gene name: Bpro_1737
Sequence domains: pfkB family carbohydrate kinase
Structure domains: UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase

Ligands and Environments

1 bound ligand:
Ligand CL 2 x CL
1 modified residue:
Ligand MSE 44 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 82.923Å b: 83.084Å c: 196.202Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.226 0.296
Expression system: Escherichia coli BL21(DE3)

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