› 4dve

X-ray diffraction
2.09Å resolution

Crystal structure at 2.1 A of the S-component for biotin from an ECF-type ABC transporter

Released:
DOI: 10.2210/pdb4dve/pdb
PDB entry 4dve coloured by chain, front view.
PDB entry 4dve coloured by chain, side view.
PDB entry 4dve coloured by chain, top view.
Source organism: Lactococcus lactis subsp. cremoris MG1363
Primary publication:
Structural divergence of paralogous S components from ECF-type ABC transporters.
Berntsson RP, ter Beek J, Majsnerowska M, Duurkens RH, Puri P, Poolman B, Slotboom DJ
Proc Natl Acad Sci U S A 109 13990-5 (2012)
PMID: 22891302

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-106951 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Biotin transporter BioY Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 198 amino acids
Theoretical weight: 21.95 KDa
Source organism: Lactococcus lactis subsp. cremoris MG1363
Expression system: Lactococcus lactis
UniProt:
  • Canonical: A2RMJ9 (Residues: 2-188; Coverage: 99%)
Gene names: bioY, llmg_1964
Sequence domains: BioY family
Structure domains: Arp2/3 complex 21 kDa subunit ARPC3

Ligands and Environments

2 bound ligands:
Ligand BTN 3 x BTN
Ligand BNG 6 x BNG
1 modified residue:
Ligand MSE 18 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2
Unit cell:
a: 89.825Å b: 57.384Å c: 166.865Å
α: 90° β: 91.07° γ: 90°
R-values:
R R work R free
0.186 0.186 0.203
Expression system: Lactococcus lactis

Quick links

Citations

4 review citations

Diversity in ABC transporters: type I, II and III importers.
Rice et al. (2014)
3 more

12 mentions without citation

Structural diversity of ABC transporters.
ter Beek et al. (2014)
11 more