› 4dw1

X-ray diffraction
2.8Å resolution

Crystal structure of the ATP-gated P2X4 ion channel in the ATP-bound, open state at 2.8 Angstroms

Released:
DOI: 10.2210/pdb4dw1/pdb
Model geometry
Fit model/data
PDB entry 4dw1 coloured by chain, front view.
PDB entry 4dw1 coloured by chain, side view.
PDB entry 4dw1 coloured by chain, top view.
Source organism: Danio rerio
Primary publication:
Molecular mechanism of ATP binding and ion channel activation in P2X receptors.
Hattori M, Gouaux E
Nature 485 207-12 (2012)
PMID: 22535247

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-244872 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
P2X purinoceptor 4a Chain: A
Molecule details ›
Chain: A
Length: 340 amino acids
Theoretical weight: 38.12 KDa
Source organism: Danio rerio
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: F8W463 (Residues: 28-365; Coverage: 87%)
Gene name: p2rx4a
Sequence domains: ATP P2X receptor
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
3 bound ligands:
Ligand NAG 1 x NAG
Ligand ATP 1 x ATP
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: R32
Unit cell:
a: 128.791Å b: 128.791Å c: 252.074Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.21 0.208 0.254
Expression system: Spodoptera frugiperda

Quick links

Citations

96 review citations

The P2X7 Receptor in Infection and Inflammation.
Di Virgilio et al. (2017)
95 more

81 mentions without citation

P2X receptors as drug targets.
North et al. (2013)
80 more