› 4dws

X-ray diffraction
1.8Å resolution

Crystal Structure of a chitinase from the Yersinia entomophaga toxin complex

Released:
DOI: 10.2210/pdb4dws/pdb
PDB entry 4dws coloured by chain, front view.
PDB entry 4dws coloured by chain, side view.
PDB entry 4dws coloured by chain, top view.
Source organism: Yersinia entomophaga
Primary publication:
The BC component of ABC toxins is an RHS-repeat-containing protein encapsulation device.
Busby JN, Panjikar S, Landsberg MJ, Hurst MR, Lott JS
Nature 501 547-50 (2013)
PMID: 23913273

Function and Biology Details

Reaction catalysed: 
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110307 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Chitinase 2 Chain: A
Molecule details ›
Chain: A
Length: 546 amino acids
Theoretical weight: 60.39 KDa
Source organism: Yersinia entomophaga
Expression system: Escherichia coli
UniProt:
  • Canonical: B6A879 (Residues: 92-633; Coverage: 86%)
Gene name: chi2
Sequence domains: Glycosyl hydrolases family 18
Structure domains:
Chitinase 2 Chain: B
Molecule details ›
Chain: B
Length: 546 amino acids
Theoretical weight: 60.32 KDa
Source organism: Yersinia entomophaga
Expression system: Escherichia coli
UniProt:
  • Canonical: B6A879 (Residues: 92-633; Coverage: 86%)
Gene name: chi2
Sequence domains: Glycosyl hydrolases family 18
Structure domains:
Chitinase 2 Chain: C
Molecule details ›
Chain: C
Length: 546 amino acids
Theoretical weight: 60.36 KDa
Source organism: Yersinia entomophaga
Expression system: Escherichia coli
UniProt:
  • Canonical: B6A879 (Residues: 92-633; Coverage: 86%)
Gene name: chi2
Sequence domains: Glycosyl hydrolases family 18
Structure domains:
Chitinase 2 Chain: D
Molecule details ›
Chain: D
Length: 546 amino acids
Theoretical weight: 60.21 KDa
Source organism: Yersinia entomophaga
Expression system: Escherichia coli
UniProt:
  • Canonical: B6A879 (Residues: 92-633; Coverage: 86%)
Gene name: chi2
Sequence domains: Glycosyl hydrolases family 18
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GOL 4 x GOL
3 modified residues:
Ligand MLY 25 x MLY
Ligand M3L 13 x M3L
Ligand MLZ 7 x MLZ

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P21
Unit cell:
a: 60.505Å b: 210.556Å c: 92.584Å
α: 90° β: 95.27° γ: 90°
R-values:
R R work R free
0.162 0.16 0.199
Expression system: Escherichia coli

Quick links

Citations

13 review citations

A view to a kill: the bacterial type VI secretion system.
Ho et al. (2014)
12 more

1 mention without citation

Cryo-EM structures of the pore-forming A subunit from the Yersinia entomophaga ABC toxin.
Piper et al. (2019)