› 4dx8

X-ray diffraction
2.54Å resolution

ICAP1 in complex with KRIT1 N-terminus

Released:
DOI: 10.2210/pdb4dx8/pdb
PDB entry 4dx8 coloured by chain, front view.
PDB entry 4dx8 coloured by chain, side view.
PDB entry 4dx8 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Mechanism for KRIT1 release of ICAP1-mediated suppression of integrin activation.
Liu W, Draheim KM, Zhang R, Calderwood DA, Boggon TJ
Mol Cell 49 719-29 (2013)
PMID: 23317506

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126333 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Integrin beta-1-binding protein 1 Chains: A, B, D, E
Molecule details ›
Chains: A, B, D, E
Length: 154 amino acids
Theoretical weight: 16.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O14713 (Residues: 49-200; Coverage: 76%)
Gene names: ICAP1, ITGB1BP1
Sequence domains: Beta-1 integrin binding protein
Structure domains: Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)
Krev interaction trapped protein 1 Chains: H, I, J, K
Molecule details ›
Chains: H, I, J, K
Length: 203 amino acids
Theoretical weight: 22.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O00522 (Residues: 1-198; Coverage: 27%)
Gene names: CCM1, KRIT1
Sequence domains: NUDIX, or N-terminal NPxY motif-rich, region of KRIT
Structure domains: KRIT, N-terminal Nudix domain, NPxY motif-rich region

Ligands and Environments

1 bound ligand:
Ligand BR 16 x BR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: C2221
Unit cell:
a: 153.898Å b: 157.977Å c: 152.006Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.22 0.252
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

26 review citations

Talins and kindlins: partners in integrin-mediated adhesion.
Calderwood et al. (2013)
25 more

2 mentions without citation

Structural basis for the disruption of the cerebral cavernous malformations 2 (CCM2) interaction with Krev interaction trapped 1 (KRIT1) by disease-associated mutations.
Fisher et al. (2015)
1 more