› 4dxe

X-ray diffraction
2.51Å resolution

2.52 Angstrom resolution crystal structure of the acyl-carrier-protein synthase (AcpS)-acyl carrier protein (ACP) protein-protein complex from Staphylococcus aureus subsp. aureus COL

Released:
DOI: 10.2210/pdb4dxe/pdb
PDB entry 4dxe coloured by chain, front view.
PDB entry 4dxe coloured by chain, side view.
PDB entry 4dxe coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus COL
Entry authors: Halavaty AS, Minasov G, Filippova EV, Dubrovska I, Winsor J, Shuvalova L, Peterson SN, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
CoA-(4'-phosphopantetheine) + an apo-[acyl-carrier-protein] = adenosine 3',5'-bisphosphate + an [acyl-carrier-protein]
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177183 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Holo-[acyl-carrier-protein] synthase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 143 amino acids
Theoretical weight: 16.37 KDa
Source organism: Staphylococcus aureus subsp. aureus COL
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5HED0 (Residues: 1-119; Coverage: 100%)
Gene names: SACOL2061, acpS
Sequence domains: 4'-phosphopantetheinyl transferase superfamily
Structure domains: 4'-phosphopantetheinyl transferase domain
Acyl carrier protein Chains: G, H, I, J, K, L
Molecule details ›
Chains: G, H, I, J, K, L
Length: 101 amino acids
Theoretical weight: 11.3 KDa
Source organism: Staphylococcus aureus subsp. aureus COL
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5HGK0 (Residues: 1-77; Coverage: 100%)
Gene names: SACOL1247, acpP
Sequence domains: Phosphopantetheine attachment site

Ligands and Environments

1 bound ligand:
Ligand MLI 4 x MLI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P21
Unit cell:
a: 76.968Å b: 102.961Å c: 77.298Å
α: 90° β: 115.87° γ: 90°
R-values:
R R work R free
0.203 0.2 0.254
Expression system: Escherichia coli

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