› 4dxm

X-ray diffraction
1.4Å resolution

Crystal Structure of an ancestral GFP-like protein

Released:
DOI: 10.2210/pdb4dxm/pdb
PDB entry 4dxm coloured by chain, front view.
PDB entry 4dxm coloured by chain, side view.
PDB entry 4dxm coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
A hinge migration mechanism unlocks the evolution of green-to-red photoconversion in GFP-like proteins.
Kim H, Zou T, Modi C, Dörner K, Grunkemeyer TJ, Chen L, Fromme R, Matz MV, Ozkan SB, Wachter RM
Structure 23 34-43 (2015)
PMID: 25565105

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164402 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GREEN FLUORESCENT PROTEIN Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 229 amino acids
Theoretical weight: 26.71 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21(DE3)
Structure domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
1 modified residue:
Ligand CR8 4 x CR8

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: C2
Unit cell:
a: 106.28Å b: 92.71Å c: 89.06Å
α: 90° β: 93.18° γ: 90°
R-values:
R R work R free
0.143 0.143 0.179
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Integration of structural dynamics and molecular evolution via protein interaction networks: a new era in genomic medicine.
Kumar et al. (2015)
3 more

1 mention without citation

To twist or not to twist: From chromophore structure to dynamics inside engineered photoconvertible and photoswitchable fluorescent proteins.
Krueger et al. (2023)