› 4dxo

X-ray diffraction
2.5Å resolution

Crystal Structure of a reconstructed Kaede-type Red Fluorescent Protein, LEA X(6)

Released:
DOI: 10.2210/pdb4dxo/pdb
PDB entry 4dxo coloured by chain, front view.
PDB entry 4dxo coloured by chain, side view.
PDB entry 4dxo coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
A hinge migration mechanism unlocks the evolution of green-to-red photoconversion in GFP-like proteins.
Kim H, Zou T, Modi C, Dörner K, Grunkemeyer TJ, Chen L, Fromme R, Matz MV, Ozkan SB, Wachter RM
Structure 23 34-43 (2015)
PMID: 25565105

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164404 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
LEA X(6) GFP-LIKE PROTEINS Chain: A
Molecule details ›
Chain: A
Length: 228 amino acids
Theoretical weight: 26.4 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21(DE3)
Structure domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
Ligand NA 1 x NA
1 modified residue:
Ligand CR8 1 x CR8

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: I222
Unit cell:
a: 47.65Å b: 78.34Å c: 119.43Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.187 0.253
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Integration of structural dynamics and molecular evolution via protein interaction networks: a new era in genomic medicine.
Kumar et al. (2015)
3 more