› 4dzp

X-ray diffraction
1.8Å resolution

Crystal structure of the terminase small subunit gp1 with R48A mutation of the bacterial virus sf6

Released:
DOI: 10.2210/pdb4dzp/pdb
PDB entry 4dzp coloured by chain, front view.
PDB entry 4dzp coloured by chain, side view.
PDB entry 4dzp coloured by chain, top view.
Source organism: Shigella virus Sf6
Primary publication:
Structural and functional studies of the phage Sf6 terminase small subunit reveal a DNA-spooling device facilitated by structural plasticity.
Zhao H, Kamau YN, Christensen TE, Tang L
J Mol Biol 423 413-26 (2012)
PMID: 22858866

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180731 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Gene 1 protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 140 amino acids
Theoretical weight: 15.49 KDa
Source organism: Shigella virus Sf6
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q716H4 (Residues: 1-140; Coverage: 100%)
Gene name: 1
Sequence domains: Bacteriophage Sf6, terminase small subunit-like
Structure domains: Homeodomain-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P4212
Unit cell:
a: 89.06Å b: 89.06Å c: 72.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.209 0.252
Expression system: Escherichia coli BL21(DE3)

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Citations

5 review citations

Molecular architecture of tailed double-stranded DNA phages.
Fokine et al. (2014)
4 more

1 mention without citation

Structural and functional studies of the phage Sf6 terminase small subunit reveal a DNA-spooling device facilitated by structural plasticity.
Zhao et al. (2012)