› 4e07

X-ray diffraction
2.9Å resolution

ParF-AMPPCP-C2221 form

Released:
DOI: 10.2210/pdb4e07/pdb
PDB entry 4e07 coloured by chain, front view.
PDB entry 4e07 coloured by chain, side view.
PDB entry 4e07 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural mechanism of ATP-induced polymerization of the partition factor ParF: implications for DNA segregation.
Schumacher MA, Ye Q, Barge MT, Zampini M, Barillà D, Hayes F
J Biol Chem 287 26146-54 (2012)
PMID: 22674577

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-109275 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CobQ/CobB/MinD/ParA nucleotide binding domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 206 amino acids
Theoretical weight: 22.06 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: B0ZE06 (Residues: 1-206; Coverage: 100%)
Gene names: CG831_004316, DNQ45_07005, EIZ93_19540, HEP30_021835, pOLA52_52, parF
Sequence domains: CobQ/CobB/MinD/ParA nucleotide binding domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand ACP 2 x ACP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: C2221
Unit cell:
a: 86.95Å b: 121.58Å c: 87.37Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.239 0.239 0.25
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Surfing biological surfaces: exploiting the nucleoid for partition and transport in bacteria.
Vecchiarelli et al. (2012)
3 more

2 mentions without citation

Structures of partition protein ParA with nonspecific DNA and ParB effector reveal molecular insights into principles governing Walker-box DNA segregation.
Zhang et al. (2017)
1 more